The Net Charge Difference Between Variant XI and Wild-type Lipase is the key question for this blog post. We will take a look at how charge changes in variant xi compare to those of wild-type lipase as well as what it means for protein stability.
The Net Charge Difference Between Variant XI and Wild-type Lipase is the key question for this blog post. We will take a look at how charge changes in variant xi compare to those of wild-type lipase as well as what it means for protein stability.
In order to tackle these questions, we must first understand what net charge is and why (or if) its important in understanding protein dynamics. Net charge can be calculated by taking the sum of all charges on each amino acid residue minus the number of neutralizing hydrogens – anything that has no polar or charged side chains such as glycine, alanine, valine, leucine are considered neutrals because they contain only carbon atoms; therefore their net
